prof. dr hab. Andriy Sybirnyy

• Biologia komórki,
• Biotechnologia drożdży,
• Drożdże niekonwencjonalne,
• Mechanizm kontrolowanej degradacji materiału komórkowego (autofagia),
• Fermentacja alkoholowa na różnych źródłach węgla (ksyloza, arabinoza),
• Produkcja ryboflawiny (witamina B2) oraz jej pochodnych (rozeoflawina, FMN, FAD),
• Produkcja glutationu oraz kwasu mlekowego przez drożdże niekonwencjnalne

• Cell biology,
• Yeast biotechnology,
• Non-conventional yeast,
• The mechanism of controlled degradation of cellular material (autophagy),
• Alcoholic fermentation on various carbon sources (xylose, arabinose),
• Production of riboflavin (vitamin B2) and its derivatives (roseoflavin, FMN, FAD),
• Production of glutathione and lactic acid by non-conventional yeast

1

Sibirny AA. Mechanisms of autophagy and pexophagy in yeasts, Biochemistry, 2011 ,76, 1279-1290, doi: 10.1134/S0006297911120017

2

Sibirny AA. Yeast peroxisomes: structure, functions and biotechnological opportunities, FEMS Yeast Res., 2016, 16, :fow038, doi: 10.1093/femsyr/fow038

3

Sibirny A. Introduction, Cell Biol Int., 2021, 45, 480, doi: 10.1002/cbin

brak

1

Sibirny AA, Shavlovsky GM. Identification of regulatory genes of riboflavin permease and α-glucosidase in the yeast Pichia guilliermondii, Curr Genet., 1984, 8, 107-114, doi: 10.1007/BF00420228

2

Sibirny AA, Titorenko VI, Gonchar MV, Ubiyvovk VM, Ksheminskaya GP, Vitvitskaya OP. Genetic control of methanol utilization in yeasts, J Basic Microbiol., 1988, 28, 293-319, doi: 10.1002/jobm.3620280503, Erratum in: J Basic Microbiol, 1989, 29, 462

3

Korpan YI, Gonchar MV, Starodub NF, Shul'ga AA, Sibirny AA, El'skaya AV. A cell biosensor specific for formaldehyde based on pH-sensitive transistors coupled to methylotrophic yeast cells with genetically adjusted metabolism, Anal Biochem., 1993, 215, 216-222, doi: 10.1006/abio.1993.1578

4

Maidan NN, Gonchar MV, Sibirny AA. Oxidation of exogenous formaldehyde in methylotrophic and nonmethylotrophic yeast cells, Biochemistry, 1997, 62, 636-640

6

Kulachkovsky AR, Moroz OM, Sibirny AA. Impairment of peroxisome degradation in Pichia methanolica mutants defective in acetyl-CoA synthetase or isocitrate lyase, Yeast, 1997, 13, 1043-1052, doi: 10.1002/(SICI)1097-0061(19970915)13:11<1043::AID-YEA161>3.0.CO;2-E

7

Gonchar MV, Kostryk LB, Sibirny AA. Cytochrome c peroxidase from a methylotrophic yeast: physiological role and isolation, Appl Microbiol Biotechnol., 1997, 48, 454-458, doi: 10.1007/s002530051079

8

Gonchar MV, Maidan MM, Moroz OM, Woodward JR, Sibirny AA. Microbial O^2- and H₂O₂-electrode sensors for alcohol assays based on the use of permeabilized mutant yeast cells as the sensitive bioelements, Biosens Bioelectron., 1998, 13, 945-952, doi: 10.1016/s0956-5663(98)00034-7

9

Johnson MA, Waterham HR, Ksheminska GP, Fayura LR, Cereghino JL, Stasyk OV, Veenhuis M, Kulachkovsky AR, Sibirny AA, Cregg JM. Positive selection of novel peroxisome biogenesis-defective mutants of the yeast Pichia pastoris, Genetics, 1999, 151, 1379-1391, doi: 10.1093/genetics/151.4.1379

10

Korpan YI, Gonchar MV, Sibirny AA, Martelet C, El'skaya AV, Gibson TD, Soldatkin AP. Development of highly selective and stable potentiometric sensors for formaldehyde determination, Biosens Bioelectron., 2000, 15, 77-83, doi: 10.1016/s0956-5663(00)00054-3

11

Borsari M, Dikaya E, Dikiy A, Gonchar MV, Maidan MM, Pierattelli R, Sibirny AA. Isolation and physico-chemical characterization of a cytochrome c from the methylotrophic yeast Hansenula polymorpha. Biochim Biophys Acta., 2000, 1543, 174-188, doi: 10.1016/s0167-4838(00)00174-6

12

Moroz OM, Gonchar MV, Sibirny AA. Efficient bioconversion of ethanol to acetaldehyde using a novel mutant strain of the methylotrophic yeast Hansenula polymorpha, Biotechnol Bioeng., 2000, 68, 44-51, doi: 10.1002/(sici)1097-0290(20000405)68:1<44::aid-bit5>3.0.co;2-8

13

Ubiyvovk VM, Nazarko TY, Stasyk OG, Sohn MJ, Kang HA, Sibirny AA. GSH2, a gene encoding gamma-glutamylcysteine synthetase in the methylotrophic yeast Hansenula polymorpha, FEMS Yeast Res., 2002, 2, 327-332, doi: 10.1016/S1567-1356(02)00111-3

14

Voronovsky AA, Abbas CA, Fayura LR, Kshanovska BV, Dmytruk KV, Sybirna KA, Sibirny AA. Development of a transformation system for the flavinogenic yeast Candida famata, FEMS Yeast Res., 2002, 2, 381-388, doi: 10.1016/S1567-1356(02)00112-5

15

Gonchar M, Maidan M, Korpan Y, Sibirny V, Kotylak Z, Sibirny A. Metabolically engineered methylotrophic yeast cells and enzymes as sensor biorecognition elements, FEMS Yeast Res., 2002 2,307-14. doi: 10.1016/S1567-1356(02)00124-1

16

Ubiyvovk VM, Maszewski J, Bartosz G, Sibirny AA. Vacuolar accumulation and extracellular extrusion of electrophilic compounds by wild-type and glutathione-deficient mutants of the methylotrophic yeast Hansenula polymorpha, Cell Biol Int., 2003, 27, 785-789, doi: 10.1016/s1065-6995(03)00127-6

17

Stasyk OV, Nazarko TY, Stasyk OG, Krasovska OS, Warnecke D, Nicaud JM, Cregg JM, Sibirny AA. Sterol glucosyltransferases have different functional roles in Pichia pastoris and Yarrowia lipolytica, Cell Biol Int., 2003, 27, 947-952, doi: 10.1016/j.cellbi.2003.08.004

18

Ryabova OB, Chmil OM, Sibirny AA. Xylose and cellobiose fermentation to ethanol by the thermotolerant methylotrophic yeast Hansenula polymorpha, FEMS Yeast Res., 2003, 4, 157-164, doi: 10.1016/S1567-1356(03)00146-6

19

Stasyk OV, Nazarko VY, Pochapinsky OD, Nazarko TY, Veenhuis M, Sibirny AA. Identification of intragenic mutations in the Hansenula polymorpha PEX6 gene that affect peroxisome biogenesis and methylotrophic growth, FEMS Yeast Res., 2003, 4,141-7, doi: 10.1016/S1567-1356(03)00153-3

20

Klionsky DJ, Cregg JM, Dunn WA Jr, Emr SD, Sakai Y, Sandoval IV, Sibirny A, Subramani S, Thumm M, Veenhuis M, Ohsumi Y. A unified nomenclature for yeast autophagy-related genes, Dev Cell., 2003, 5, 539-45, doi: 10.1016/s1534-5807(03)00296-x

21

Voronovsky AY, Abbas CA, Dmytruk KV, Ishchuk OP, Kshanovska BV, Sybirna KA, Gaillardin C, Sibirny AA. Candida famata (Debaryomyces hansenii) DNA sequences containing genes involved in riboflavin synthesis, Yeast, 2004, 21, 1307-16, doi: 10.1002/yea.1182

22

Stasyk OV, Stasyk OG, Komduur J, Veenhuis M, Cregg JM, Sibirny AA. A hexose transporter homologue controls glucose repression in the methylotrophic yeast Hansenula polymorpha, J Biol Chem., 2004, 279, 8116-25, doi: 10.1074/jbc.M310960200

23

Voronovsky AY, Ryabova OB, Verba OV, Ishchuk OP, Dmytruk KV, Sibirny AA. Expression of xylA genes encoding xylose isomerases from Escherichia coli and Streptomyces coelicolor in the methylotrophic yeast Hansenula polymorpha, FEMS Yeast Res., 2005, 5, 1055-62, doi: 10.1016/j.femsyr.2005.09.001

24

Boretsky YR, Kapustyak KY, Fayura LR, Stasyk OV, Stenchuk MM, Bobak YP, Drobot LB, Sibirny AA. Positive selection of mutants defective in transcriptional repression of riboflavin synthesis by iron in the flavinogenic yeast Pichia guilliermondii, FEMS Yeast Res., 2005, 5, 829-37, doi: 10.1016/j.femsyr.2005.03.007

25

Nazarko TY, Nicaud JM, Sibirny AA. Observation of the Yarrowia lipolytica peroxisome-vacuole dynamics by fluorescence microscopy with a single filter set, Cell Biol Int., 2005, 29, 65-70, doi: 10.1016/j.cellbi.2004.11.014

26

Nazarko TY, Huang J, Nicaud JM, Klionsky DJ, Sibirny AA. Trs85 is required for macroautophagy, pexophagy and cytoplasm to vacuole targeting in Yarrowia lipolytica and Saccharomyces cerevisiae, Autophagy., 2005, 1, 37-45, doi: 10.4161/auto.1.1.1512

27

Dunn WA Jr, Cregg JM, Kiel JA, van der Klei IJ, Oku M, Sakai Y, Sibirny AA, Stasyk OV, Veenhuis M. Pexophagy: the selective autophagy of peroxisomes, Autophagy, 2005, 1, 75-83, doi: 10.4161/auto.1.2.1737

28

Gonchar MV, Grabek D, Oklejewicz B, Pavlishko HM, Shamlian OV, Sybirny VA, Kotylak Z, Rudke K, Csöregi E, Sibirny AA. A new enzymo-chemical method for simultaneous assay of methanol and formaldehyde, Ukr Biokhim Zh (1999), 2005, 77, 146-54

29

Ubiyvovk VM, Blazhenko OV, Gigot D, Penninckx M, Sibirny AA. Role of gamma-glutamyltranspeptidase in detoxification of xenobiotics in the yeasts Hansenula polymorpha and Saccharomyces cerevisiae, Cell Biol Int., 2006, 30, 665-71, doi: 10.1016/j.cellbi.2006.04.006

30

Blazhenko OV, Zimmermann M, Kang HA, Bartosz G, Penninckx MJ, Ubiyvovk VM, Sibirny AA. Accumulation of cadmium ions in the methylotrophic yeast Hansenula polymorpha, Biometals, 2006, 19, 593-9, doi: 10.1007/s10534-006-0005-0

31

Grabek-Lejko D, Ryabova OB, Oklejewicz B, Voronovsky AY, Sibirny AA. Plate ethanol-screening assay for selection of the Pichia stipitis and Hansenula polymorpha yeast mutants with altered capability for xylose alcoholic fermentation, J Ind Microbiol Biotechnol., 2006, 33, 934-40, doi: 10.1007/s10295-006-0147-7

32

Stasyk OV, Stasyk OG, Mathewson RD, Farré JC, Nazarko VY, Krasovska OS, Subramani S, Cregg JM, Sibirny AA. Atg28, a novel coiled-coil protein involved in autophagic degradation of peroxisomes in the methylotrophic yeast Pichia pastoris, Autophagy, 2006, 2, 30-8, doi: 10.4161/auto.2226

33

Dmytruk KV, Voronovsky AY, Sibirny AA. Insertion mutagenesis of the yeast Candida famata (Debaryomyces hansenii) by random integration of linear DNA fragments, Curr Genet., 2006, 50, 183-91, doi: 10.1007/s00294-006-0083-0

34

Dmytruk KV, Smutok OV, Ryabova OB, Gayda GZ, Sibirny VA, Schuhmann W, Gonchar MV, Sibirny AA. Isolation and characterization of mutated alcohol oxidases from the yeast Hansenula polymorpha with decreased affinity toward substrates and their use as selective elements of an amperometric biosensor, BMC Biotechnol., 2007, 7, 33, doi: 10.1186/1472-6750-7-33

35

Stasyk OG, van Zutphen T, Ah Kang H, Stasyk OV, Veenhuis M, Sibirny AA. The role of Hansenula polymorpha MIG1 homologues in catabolite repression and pexophagy, FEMS Yeast Res., 2007, 7, 1103-13, doi: 10.1111/j.1567-1364.2007.00286.x

36

Saloheimo A, Rauta J, Stasyk OV, Sibirny AA, Penttilä M, Ruohonen L. Xylose transport studies with xylose-utilizing Saccharomyces cerevisiae strains expressing heterologous and homologous permeases, Appl Microbiol Biotechnol., 2007, 74, 1041-52, doi: 10.1007/s00253-006-0747-1

37

Smutok O, Dmytruk K, Gonchar M, Sibirny A, Schuhmann W. Permeabilized cells of flavocytochrome b2 over-producing recombinant yeast Hansenula polymorpha as biological recognition element in amperometric lactate biosensors, Biosens Bioelectron., 2007, 23, 599-605, doi: 10.1016/j.bios.2007.06.021

38

Nazarko TY, Farré JC, Polupanov AS, Sibirny AA, Subramani S. Autophagy-related pathways and specific role of sterol glucoside in yeasts, Autophagy, 2007, 3, 263-5, doi: 10.4161/auto.3907.

39

Nazarko TY, Polupanov AS, Manjithaya RR, Subramani S, Sibirny AA. The requirement of sterol glucoside for pexophagy in yeast is dependent on the species and nature of peroxisome inducers, Mol Biol Cell., 2007, 18, 106-18, doi: 10.1091/mbc.e06-06-0554

40

Krasovska OS, Stasyk OG, Nahorny VO, Stasyk OV, Granovski N, Kordium VA, Vozianov OF, Sibirny AA. Glucose-induced production of recombinant proteins in Hansenula polymorpha mutants deficient in catabolite repression, Biotechnol Bioeng., 2007, 97, 858-70, doi: 10.1002/bit.21284

41

Boretsky YR, Pynyaha YV, Boretsky VY, Kutsyaba VI, Protchenko OV, Philpott CC, Sibirny AA. Development of a transformation system for gene knock-out in the flavinogenic yeast Pichia guilliermondii, J Microbiol Methods., 2007, 70, 13-9, doi: 10.1016/j.mimet.2007.03.004

42

Boretsky YR, Protchenko OV, Prokopiv TM, Mukalov IO, Fedorovych DV, Sibirny AA. Mutations and environmental factors affecting regulation of riboflavin synthesis and iron assimilation also cause oxidative stress in the yeast Pichia guilliermondii, J Basic Microbiol., 2007, 47, 371-7, doi: 10.1002/jobm.200610279

43

Stasyk OG, Maidan MM, Stasyk OV, Van Dijck P, Thevelein JM, Sibirny AA. Identification of hexose transporter-like sensor HXS1 and functional hexose transporter HXT1 in the methylotrophic yeast Hansenula polymorpha, Eukaryot Cell., 2008, 7, 735-46, doi: 10.1128/EC.00028-08

44

Klionsky DJ, Sibirny A, at all. Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes, Autophagy, 2008, 4, 151-75, doi: 10.4161/auto.5338

45

Nazarko VY, Futej KO, Thevelein JM, Sibirny AA. Differences in glucose sensing and signaling for pexophagy between the baker's yeast Saccharomyces cerevisiae and the methylotrophic yeast Pichia pastoris, Autophagy, 2008, 4, 381-4, doi: 10.4161/auto.5634

46

Dmytruk OV, Voronovsky AY, Abbas CA, Dmytruk KV, Ishchuk OP, Sibirny AA. Overexpression of bacterial xylose isomerase and yeast host xylulokinase improves xylose alcoholic fermentation in the thermotolerant yeast Hansenula polymorpha, FEMS Yeast Res., 2008, 8, 165-73, doi: 10.1111/j.1567-1364.2007.00289.x

47

Dmytruk OV, Dmytruk KV, Abbas CA, Voronovsky AY, Sibirny AA. Engineering of xylose reductase and overexpression of xylitol dehydrogenase and xylulokinase improves xylose alcoholic fermentation in the thermotolerant yeast Hansenula polymorpha, Microb Cell Fact., 2008, 7, 21, doi: 10.1186/1475-2859-7-21

48

Nazarko VY, Thevelein JM, Sibirny AA. G-protein-coupled receptor Gpr1 and G-protein Gpa2 of cAMP-dependent signaling pathway are involved in glucose-induced pexophagy in the yeast Saccharomyces cerevisiae, Cell Biol Int., 2008, 32, 502-4, doi: 10.1016/j.cellbi.2007.11.001

49

Lahtchev KL, Batovska DI, Parushev SP, Ubiyvovk VM, Sibirny AA. Antifungal activity of chalcones: a mechanistic study using various yeast strains, Eur J Med Chem., 2008, 43, 2220-8, doi: 10.1016/j.ejmech.2007.12.027

50

Stasyk OV, Nazarko TY, Sibirny AA. Methods of plate pexophagy monitoring and positive selection for ATG gene cloning in yeasts, Methods Enzymol., 2008, 451, 229-39, doi: 10.1016/S0076-6879(08)03216-3

51

Ishchuk OP, Voronovsky AY, Stasyk OV, Gayda GZ, Gonchar MV, Abbas CA, Sibirny AA. Overexpression of pyruvate decarboxylase in the yeast Hansenula polymorpha results in increased ethanol yield in high-temperature fermentation of xylose, FEMS Yeast Res., 2008, 8, 1164-74, doi: 10.1111/j.1567-1364.2008.00429.x

52

Ishchuk OP, Voronovsky AY, Abbas CA, Sibirny AA. Construction of Hansenula polymorpha strains with improved thermotolerance, Biotechnol Bioeng., 2009, 104, 911-9, doi: 10.1002/bit.22457

53

Voronovsky AY, Rohulya OV, Abbas CA, Sibirny AA. Development of strains of the thermotolerant yeast Hansenula polymorpha capable of alcoholic fermentation of starch and xylan, Metab Eng., 2009, 11, 234-42, doi: 10.1016/j.ymben.2009.04.001

54

Yatsyshyn VY, Ishchuk OP, Voronovsky AY, Fedorovych DV, Sibirny AA. Production of flavin mononucleotide by metabolically engineered yeast Candida famata, Metab Eng., 2009, 11, 163-7, doi: 10.1016/j.ymben.2009.01.004

55

Shkil H, Stoica L, Dmytruk K, Smutok O, Gonchar M, Sibirny A, Schuhmann W. Bioelectrochemical detection of L-lactate respiration using genetically modified Hansenula polymorpha yeast cells overexpressing flavocytochrome b2, Bioelectrochemistry, 2009, 76, 175-9, doi: 10.1016/j.bioelechem.2009.04.002

56

Pynyaha YV, Boretsky YR, Fedorovych DV, Fayura LR, Levkiv AI, Ubiyvovk VM, Protchenko OV, Philpott CC, Sibirny AA. Deficiency in frataxin homologue YFH1 in the yeast Pichia guilliermondii leads to missregulation of iron acquisition and riboflavin biosynthesis and affects sulfate assimilation, Biometals., 2009, 22, 1051-61, doi: 10.1007/s10534-009-9256-x

57

Ishchuk OP, Abbas CA, Sibirny AA. Heterologous expression of Saccharomyces cerevisiae MPR1 gene confers tolerance to ethanol and L: -azetidine-2-carboxylic acid in Hansenula polymorpha, J Ind Microbiol Biotechnol., 2010, 37, 213-8, doi: 10.1007/s10295-009-0674-0

58

Bobak YP, Vynnytska BO, Kurlishchuk YV, Sibirny AA, Stasyk OV. Cancer cell sensitivity to arginine deprivation in vitro is not determined by endogenous levels of arginine metabolic enzymes, Cell Biol Int., 2010, 34, 1085-9, doi: 10.1042/CBI20100451

59

Sybirna K, Petryk N, Zhou YF, Sibirny A, Bolotin-Fukuhara M. A novel Hansenula polymorpha transcriptional factor HpHAP4-B, able to functionally replace the S. cerevisiae HAP4 gene, contains an additional bZip motif, Yeast, 2010, 27, 941-54, doi: 10.1002/yea.1802

60

Boretsky YR, Pynyaha YV, Boretsky VY, Fedorovych DV, Fayura LR, Protchenko O, Philpott CC, Sibirny AA. Identification of the genes affecting the regulation of riboflavin synthesis in the flavinogenic yeast Pichia guilliermondii using insertion mutagenesis, FEMS Yeast Res., 2011, 11, 307-14, doi: 10.1111/j.1567-1364.2011.00720.x

61

Abbas CA, Sibirny AA. Genetic control of biosynthesis and transport of riboflavin and flavin nucleotides and construction of robust biotechnological producers, Microbiol Mol Biol Rev., 2011, 75, 321-60, doi: 10.1128/MMBR.00030-10

62

Millerioux Y, Clastre M, Simkin AJ, Courdavault V, Marais E, Sibirny AA, Noël T, Crèche J, Giglioli-Guivarc'h N, Papon N. Drug-resistant cassettes for the efficient transformation of Candida guilliermondii wild-type strains, FEMS Yeast Res., 2011, 11, 457-63, doi: 10.1111/j.1567-1364.2011.00731.x

63

Polupanov AS, Nazarko VY, Sibirny AA. CCZ1, MON1 and YPT7 genes are involved in pexophagy, the Cvt pathway and non-specific macroautophagy in the methylotrophic yeast Pichia pastoris, Cell Biol Int., 2011, 35, 311-9, doi: 10.1042/CBI20100547

64

Grabek-Lejko D, Kurylenko OO, Sibirny VA, Ubiyvovk VM, Penninckx M, Sibirny AA. Alcoholic fermentation by wild-type Hansenula polymorpha and Saccharomyces cerevisiae versus recombinant strains with an elevated level of intracellular glutathione, J Ind Microbiol Biotechnol., 2011, 38, 1853-9, doi: 10.1007/s10295-011-0974-z

65

Ubiyvovk VM, Ananin VM, Malyshev AY, Kang HA, Sibirny AA. Optimization of glutathione production in batch and fed-batch cultures by the wild-type and recombinant strains of the methylotrophic yeast Hansenula polymorpha DL-1, BMC Biotechnol., 2011, 11, 8, doi: 10.1186/1472-6750-11-8

66

Nazarko VY, Nazarko TY, Farré JC, Stasyk OV, Warnecke D, Ulaszewski S, Cregg JM, Sibirny AA, Subramani S. Atg35, a micropexophagy-specific protein that regulates micropexophagic apparatus formation in Pichia pastoris, Autophagy, 2011, 7, 375-85, doi: 10.4161/auto.7.4.14369

67

Dmytruk KV, Smutok OV, Dmytruk OV, Schuhmann W, Sibirny AA. Construction of uricase-overproducing strains of Hansenula polymorpha and its application as biological recognition element in microbial urate biosensor, BMC Biotechnol., 2011, 11, 58, doi: 10.1186/1472-6750-11-58

68

Dmytruk KV, Sibirny AA. Candida famata (Candida flareri), Yeast, 2012, 29, 453-8, doi: 10.1002/yea.2929

69

Polupanov AS, Nazarko VY, Sibirny AA. Gss1 protein of the methylotrophic yeast Pichia pastoris is involved in glucose sensing, pexophagy and catabolite repression, Int J Biochem Cell Biol., 2012, 44, 1906-18, doi: 10.1016/j.biocel.2012.07.017

70

Klionsky DJ, Sibirny AA, et al. Guidelines for the use and interpretation of assays for monitoring autophagy, Autophagy, 2012, 8, 445-544, doi: 10.4161/auto.19496

71

Foureau E, Courdavault V, Simkin AJ, Sibirny AA, Crèche J, Giglioli-Guivarc'h N, Clastre M, Papon N. Transformation of Candida guilliermondii wild-type strains using the Staphylococcus aureus MRSA 252 ble gene as a phleomycin-resistant marker, FEMS Yeast Res., 2013, 13, 354-8, doi: 10.1111/1567-1364.12034

72

Krasovska OS, Stasyk OV, Sibirny AA. Stable overproducer of hepatitis B surface antigen in the methylotrophic yeast Hansenula polymorpha due to multiple integration of heterologous auxotrophic selective markers and defect in peroxisome biogenesis, Appl Microbiol Biotechnol., 2013, 97(23):9969-79. doi: 10.1007/s00253-013-5223-0

73

Grabek-Lejko D, Sibirny V, Sibirny A. Regulacja ekspresji genów w komórkach drozdzy metylotroficznych [Regulation of gene expression in methylotrophic yeasts], Postepy Biochem., 2013, 59, 95-106

74

Papon N, Savini V, Lanoue A, Simkin AJ, Crèche J, Giglioli-Guivarc'h N, Clastre M, Courdavault V, Sibirny AA. Candida guilliermondii: biotechnological applications, perspectives for biological control, emerging clinical importance and recent advances in genetics, Curr Genet., 2013, 59, 73-90, doi: 10.1007/s00294-013-0391-0

75

Prokopiv TM, Fedorovych DV, Boretsky YR, Sibirny AA. Oversynthesis of riboflavin in the yeast Pichia guilliermondii is accompanied by reduced catalase and superoxide dismutases activities, Curr Microbiol., 2013, 66, 79-87, doi: 10.1007/s00284-012-0242-0

76

Fayura LR, Boretsky YR, Pynyaha YV, Wheatley DN, Sibirny AA. Improved method for expression and isolation of the Mycoplasma hominis arginine deiminase from the recombinant strain of Escherichia coli. J Biotechnol., 2013, 167, 420-6, doi: 10.1016/j.jbiotec.2013.06.025

77

Polupanov AS, Sibirny AA. Cytoplasmic extension peptide of Pichia pastoris glucose sensor Gss1 is not compulsory for glucose signalling, Cell Biol Int., 2014, 38, 172-8, doi: 10.1002/cbin.10189

78

Yatsyshyn VY, Fedorovych DV, Sibirny AA. Metabolic and bioprocess engineering of the yeast Candida famata for FAD production, J Ind Microbiol Biotechnol., 2014, 41, 823-35, doi: 10.1007/s10295-014-1422-7

79

Sohn MJ, Yoo SJ, Oh DB, Kwon O, Lee SY, Sibirny AA, Kang HA. Novel cysteine-centered sulfur metabolic pathway in the thermotolerant methylotrophic yeast Hansenula polymorpha, PLoS One., 2014, 9, e100725, doi: 10.1371/journal.pone.0100725

80

Smutok OV, Dmytruk KV, Karkovska MI, Schuhmann W, Gonchar MV, Sibirny AA. d-lactate-selective amperometric biosensor based on the cell debris of the recombinant yeast Hansenula polymorpha, Talanta, 2014, 125, 227-32, doi: 10.1016/j.talanta.2014.02.041

81

Kurylenko OO, Ruchala J, Hryniv OB, Abbas CA, Dmytruk KV, Sibirny AA. Metabolic engineering and classical selection of the methylotrophic thermotolerant yeast Hansenula polymorpha for improvement of high-temperature xylose alcoholic fermentation, Microb Cell Fact., 2014, 13, 122, doi: 10.1186/s12934-014-0122-3

82

Semkiv MV, Dmytruk KV, Abbas CA, Sibirny AA. Increased ethanol accumulation from glucose via reduction of ATP level in a recombinant strain of Saccharomyces cerevisiae overexpressing alkaline phosphatase, BMC Biotechnol., 2014, 14, 42, doi: 10.1186/1472-6750-14-42

83

Rachkevych N, Sybirna K, Boyko S, Boretsky Y, Sibirny A. Improving the efficiency of plasmid transformation in Shewanella oneidensis MR-1 by removing ClaI restriction site, J Microbiol Methods., 2014, 99, 35-7, doi: 10.1016/j.mimet.2014.01.009

84

Dmytruk K, Lyzak O, Yatsyshyn V, Kluz M, Sibirny V, Puchalski C, Sibirny A. Construction and fed-batch cultivation of Candida famata with enhanced riboflavin production, J Biotechnol., 2014, 172, 11-7, doi: 10.1016/j.jbiotec.2013.12.005

85

Petryk N, Zhou YF, Sybirna K, Mucchielli MH, Guiard B, Bao WG, Stasyk OV, Stasyk OG, Krasovska OS, Budin K, Reymond N, Imbeaud S, Coudouel S, Delacroix H, Sibirny A, Bolotin-Fukuhara M. Functional study of the Hap4-like genes suggests that the key regulators of carbon metabolism HAP4 and oxidative stress response YAP1 in yeast diverged from a common ancestor, PLoS One, 2014, 9, e112263, doi: 10.1371/journal.pone.0112263

86

Tangsombatvichit P, Semkiv MV, Sibirny AA, Jensen LT, Ratanakhanokchai K, Soontorngun N. Zinc cluster protein Znf1, a novel transcription factor of non-fermentative metabolism in Saccharomyces cerevisiae, FEMS Yeast Res., 2015, 15, fou002, doi: 10.1093/femsyr/fou002

87

Stasyk OV, Boretsky YR, Gonchar MV, Sibirny AA. Recombinant arginine-degrading enzymes in metabolic anticancer therapy and bioanalytics, Cell Biol Int., 2015, 39, 246-52, doi: 10.1002/cbin.10383

88

Klionsky DJ, Sibirny AA et al. Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition), Autophagy, 2016, 12, 1-222, doi: 10.1080/15548627.2015.1100356, Erratum in: Autophagy, 2016, 12, 443

89

Jansuriyakul S, Somboon P, Rodboon N, Kurylenko O, Sibirny A, Soontorngun N. The zinc cluster transcriptional regulator Asg1 transcriptionally coordinates oleate utilization and lipid accumulation in Saccharomyces cerevisiae, Appl Microbiol Biotechnol., 2016, 100, 4549-60, doi: 10.1007/s00253-016-7356-4

90

Ishchuk OP, Vojvoda Zeljko T, Schifferdecker AJ, Mebrahtu Wisén S, Hagström ÅK, Rozpędowska E, Rørdam Andersen M, Hellborg L, Ling Z, Sibirny AA, Piškur J. Novel Centromeric Loci of the Wine and Beer Yeast Dekkera bruxellensis CEN1 and CEN2, PLoS One, 2016, 11, e0161741,  doi: 10.1371/journal.pone.0161741

91

Stasyuk NY, Gayda GZ, Fayura LR, Boretskyy YR,  Gonchar M, Sibirny AA. Novel arginine deiminase-based method to assay L-arginine in beverages. Food Chem., 2016, 201:320-6. doi: 10.1016/j.foodchem.2016.01.093

92

Kata I, Semkiv MV, Ruchala J, Dmytruk KV, Sibirny AA. Overexpression of the genes PDC1 and ADH1 activates glycerol conversion to ethanol in the thermotolerant yeast Ogataea (Hansenula) polymorpha, Yeast, 2016, 33, 471-8, doi: 10.1002/yea.3175

93

Semkiv MV, Dmytruk KV, Sibirny AA. Development of a system for multicopy gene integration in Saccharomyces cerevisiae, J Microbiol Methods., 2016, 120, 44-9, doi: 10.1016/j.mimet.2015.10.023

94

Semkiv MV, Dmytruk KV, Abbas CA, Sibirny AA. Activation of futile cycles as an approach to increase ethanol yield during glucose fermentation in Saccharomyces cerevisiae, Bioengineered, 2016,7, 106-11, doi: 10.1080/21655979.2016.1148223

95

Schifferdecker AJ, Siurkus J, Andersen MR, Joerck-Ramberg D, Ling Z, Zhou N, Blevins JE, Sibirny AA, Piškur J, Ishchuk OP. Erratum to: Alcohol dehydrogenase gene ADH3 activates glucose alcoholic fermentation in genetically engineered Dekkera bruxellensis yeast, Appl Microbiol Biotechnol., 2016, 100, 3233, doi: 10.1007/s00253-016-7349-3, Erratum for: Appl Microbiol Biotechnol., 2016, 100, 3219-31

96

Schifferdecker AJ, Siurkus J, Andersen MR, Joerck-Ramberg D, Ling Z, Zhou N, Blevins JE, Sibirny AA, Piškur J, Ishchuk OP. Alcohol dehydrogenase gene ADH3 activates glucose alcoholic fermentation in genetically engineered Dekkera bruxellensis yeast, Appl Microbiol Biotechnol., 2016, 100, 3219-31, doi: 10.1007/s00253-015-7266-x, Erratum in: Appl Microbiol Biotechnol., 2016, 100, 3233

97

Riley R, Haridas , Wolfe KH, Lopes MR.,Hittinger CT, Göker M, Salamov AA, Wisecaver JH, Long TM, Calvey CH, Aerts AL, Barry KW, Choi C, Clum A, Coughlan AY, Deshpande S, Douglass AP, Hanson SJ, Klenk HP, LaButti KM, Lapidus A, Lindquist EA, Lipzen AM, Meier-Kolthoff JP, Ohm RA, Otillar RP, Pangilinan JL, Peng Y, Rokas A, Rosa CA, Scheuner C, Sibirny AA, Slot JC, Stielow JB, Sun H, Kurtzman CP, Blackwell M, Grigoriev IV, Jeffries TW. Comparative genomics of biotechnologically important yeasts, Proc Natl Acad Sci USA, 2016, 113, 9882-7, doi: 10.1073/pnas.1603941113

98

Murashchenko L, Abbas C, Dmytruk K, Sibirny A. Overexpression of the truncated version of ILV2 enhances glycerol production in Saccharomyces cerevisiae, Yeast, 2016, 33, 463-9, doi: 10.1002/yea.3161

99

Kurylenko O, Semkiv M, Ruchala J, Hryniv O, Kshanovska B, Abbas C, Dmytruk K, Sibirny A. New approaches for improving the production of the 1st and 2nd generation ethanol by yeast. Acta Biochim Pol., 2016, 63, 31-38, doi: 10.18388/abp.2015_1156

100

Semkiv MV, Dmytruk KV, Abbas CA, Sibirny AA. Metabolic engineering for high glycerol production by the anaerobic cultures of Saccharomyces cerevisiae, Appl Microbiol Biotechnol., 2017, 101, 4403-4416, doi: 10.1007/s00253-017-8202-z

101

Ruchala J, Kurylenko OO, Soontorngun N, Dmytruk KV, Sibirny AA. Transcriptional activator Cat8 is involved in regulation of xylose alcoholic fermentation in the thermotolerant yeast Ogataea (Hansenula) polymorpha, Microb Cell Fact., 2017, 16, 36, doi: 10.1186/s12934-017-0652-6

102

Farré JC, Carolino K, Stasyk OV, Stasyk OG, Hodzic Z, Agrawal G, Till A, Proietto M, Cregg J, Sibirny AA, Subramani S, A New Yeast Peroxin, Pex36, a Functional Homolog of Mammalian PEX16, Functions in the ER-to-Peroxisome Traffic of Peroxisomal Membrane Proteins, J Mol Biol., 2017, 429, 3743-3762, doi: 10.1016/j.jmb.2017.10.009

103

Dmytruk KV, Ruchala J, Grabek-Lejko D, Puchalski C, Bulbotka NV, Sibirny AA. Autophagy-related gene ATG13 is involved in control of xylose alcoholic fermentation in the thermotolerant methylotrophic yeast Ogataea polymorpha, FEMS Yeast Res., 2018, 18, doi: 10.1093/femsyr/foy010

104

Yurkiv M, Kurylenko O, Vasylyshyn R, Dmytruk K, Fickers P, Sibirny A. Gene of the transcriptional activator MET4 is involved in regulation of glutathione biosynthesis in the methylotrophic yeast Ogataea (Hansenula) polymorpha, FEMS Yeast Res., 2018, 18, doi: 10.1093/femsyr/foy004

105

Kurylenko OO, Ruchala J, Vasylyshyn RV, Stasyk OV, Dmytruk OV, Dmytruk KV, Sibirny AA. Peroxisomes and peroxisomal transketolase and transaldolase enzymes are essential for xylose alcoholic fermentation by the methylotrophic thermotolerant yeast, Ogataea (Hansenula) polymorpha, Biotechnol Biofuels, 2018, 11, 197, doi: 10.1186/s13068-018-1203-z

106

Wang M, Cao Y, Xia M, Al-Hatmi AMS, Ou W, Wang Y, Sibirny AA, Zhao L, Zou C, Liao W, Bai F, Zhi X, de Hoog S, Kang Y. Virulence and antifungal susceptibility of microsatellite genotypes of Candida albicans from superficial and deep locations, Yeast, 2019, 36, 363-373, doi: 10.1002/yea.3397

107

Kurylenko O, Rozenfelde L, Khroustalyova G, Vasylyshyn R, Ruchala J, Chang CR, Daugelavicius R, Sibirny A. Rapoport A. Anhydrobiosis in yeasts: Glutathione synthesis by yeast Ogataea (Hansenula) polymorpha cells after their dehydration-rehydration, J Biotechnol., 2019, 304, 28-30, doi: 10.1016/j.jbiotec.2019.08.005

108

Semkiv M, Kata I, Ternavska O, Sibirny W, Dmytruk K, Sibirny A. Overexpression of the genes of glycerol catabolism and glycerol facilitator improves glycerol conversion to ethanol in the methylotrophic thermotolerant yeast Ogataea polymorpha, Yeast, 2019, 36, 329-339, doi: 10.1002/yea.3387

109

Vasylyshyn R, Kurylenko O, Ruchala J, Shevchuk N, Kuliesiene N, Khroustalyova G, Rapoport A, Daugelavicius R, Dmytruk K, Sibirny A. Engineering of sugar transporters for improvement of xylose utilization during high-temperature alcoholic fermentation in Ogataea polymorpha yeast, Microb Cell Fact., 2020, 19, 96, doi: 10.1186/s12934-020-01354-9

110

Tsyrulnyk AO, Andreieva YA, Ruchala J, Fayura LR, Dmytruk KV, Fedorovych DV, Sibirny AA. Expression of yeast homolog of the mammal BCRP gene coding for riboflavin efflux protein activates vitamin B2 production in the flavinogenic yeast Candida famata, Yeast, 2020, 37, 467-473, doi: 10.1002/yea.3470

111

Songdech P, Ruchala J, Semkiv MV, Jensen LT, Sibirny A, Ratanakhanokchai K, Soontorngun N. Overexpression of Transcription Factor ZNF1 of Glycolysis Improves Bioethanol Productivity under High Glucose Concentration and Enhances Acetic Acid Tolerance of Saccharomyces cerevisiae, Biotechnol J., 2020, 15, e1900492, doi: 10.1002/biot.201900492

112

Ruchala J, Kurylenko OO, Dmytruk KV, Sibirny AA. Construction of advanced producers of first- and second-generation ethanol in Saccharomyces cerevisiae and selected species of non-conventional yeasts (Scheffersomyces stipitis, Ogataea polymorpha), J Ind Microbiol Biotechnol, 2020, 47, 109-132, doi: 10.1007/s10295-019-02242-x

113

Kurylenko OO, Ruchala J, Dmytruk KV, Abbas CA, Sibirny AA. Multinuclear Yeast Magnusiomyces (Dipodascus, Endomyces) magnusii is a Promising Isobutanol Producer, Biotechnol J., 2020, 15, e1900490, doi: 10.1002/biot.201900490

114

Dzanaeva L, Kruk B, Ruchala J, Nielsen J, Sibirny A, Dmytruk K. The role of peroxisomes in xylose alcoholic fermentation in the engineered Saccharomyces cerevisiae, Cell Biol Int., 2020, 44, 1606-1615, doi: 10.1002/cbin.11353

115

Dmytruk KV, Ruchala J, Fedorovych DV, Ostapiv RD, Sibirny AA. Modulation of the Purine Pathway for Riboflavin Production in Flavinogenic Recombinant Strain of the Yeast Candida famata, Biotechnol J., 2020, 15, e1900468, doi: 10.1002/biot.201900468

116

Bratiichuk D, Kurylenko O, Vasylyshyn R, Zuo M, Kang Y, Dmytruk K, Sibirny A. Development of new dominant selectable markers for the nonconventional yeasts Ogataea polymorpha and Candida famata, Yeast, 2020, 37, 505-513, doi: 10.1002/yea.3467

117

Andreieva Y, Petrovska Y, Lyzak O, Liu W, Kang Y, Dmytruk K, Sibirny A. Role of the regulatory genes SEF1, VMA1 and SFU1 in riboflavin synthesis in the flavinogenic yeast Candida famata (Candida flareri), Yeast, 2020, 37, 497-504, doi: 10.1002/yea.3503

118

Ruchala J, Sibirny AA. Pentose metabolism and conversion to biofuels and high-value chemicals in yeasts, FEMS Microbiol Rev., 2021, 4, fuaa069, doi: 10.1093/femsre/fuaa069

119

Dmytruk O, Bulbotka N, Zazulya A, Semkiv M, Dmytruk K, Sibirny A. Fructose-1,6-bisphosphatase degradation in the methylotrophic yeast Komagataella phaffii occurs in autophagy pathway, Cell Biol Int., 2021, 45, 528-535, doi: 10.1002/cbin.11304

120

Berezka K, Semkiv M, Borbuliak M, Blomqvist J, Linder T, Ruchała J, Dmytruk K, Passoth V, Sibirny A. Insertional tagging of the Scheffersomyces stipitis gene HEM25 involved in regulation of glucose and xylose alcoholic fermentation, Cell Biol Int., 2021, 45, 507-517, doi: 10.1002/cbin.11284

121

Kurylenko O, Ruchala J, Kruk B, Vasylyshyn R, Szczepaniak J, Dmytruk K, Sibirny A. The role of Mig1, Mig2, Tup1 and Hap4 transcription factors in regulation of xylose and glucose fermentation in the thermotolerant yeast Ogataea polymorpha, FEMS Yeast Res., 2021, 21, foab029, doi: 10.1093/femsyr/foab029

122

Dzanaeva L, Kruk B, Ruchala J, Sibirny A, Dmytruk K. The impact of transcription factors Znf1, Sip4, Adr1, Tup1, and Hap4 on xylose alcoholic fermentation in the engineered yeast Saccharomyces cerevisiae, Antonie Van Leeuwenhoek, 2021, 114, 1373-1385, doi: 10.1007/s10482-021-01607-6

123

Fedorovych DV, Dmytruk KV, Sibirny AA. Recent Advances in Construction of the Efficient Producers of Riboflavin and Flavin Nucleotides (FMN, FAD) in the Yeast Candida famata, Methods Mol Biol., 2021, 2280, 15-30, doi: 10.1007/978-1-0716-1286-6_2

124

Tsyrulnyk AO, Fedorovych DV, Dmytruk KV, Sibirny AA. Overexpression of Riboflavin Excretase Enhances Riboflavin Production in the Yeast Candida famata, Methods Mol Biol. 2021, 2280, 31-42, doi: 10.1007/978-1-0716-1286-6_3

125

Smutok OV, Dmytruk KV, Kavetskyy TS, Sibirny AA, Gonchar MV. Flavocytochrome b2 of the Methylotrophic Yeast Ogataea polymorpha: Construction of Overproducers, Purification, and Bioanalytical Application, Methods Mol Biol., 2021, 2280, 249-260,doi: 10.1007/978-1-0716-1286-6_16

126

Fedorovych DV, Dmytruk KV, Sibirny AA. Recent Advances in Construction of the Efficient Producers of Riboflavin and Flavin Nucleotides (FMN, FAD) in the Yeast Candida famata, Methods Mol Biol., 2021, 2280, 15-30, doi: 10.1007/978-1-0716-1286-6_2

127

Semkiv MV, Ruchala J, Tsaruk AY, Zazulya AZ, Vasylyshyn RV, Dmytruk OV, Zuo M, Kang Y, Dmytruk KV, Sibirny AA. The role of hexose transporter-like sensor hxs1 and transcription activator involved in carbohydrate sensing azf1 in xylose and glucose fermentation in the thermotolerant yeast Ogataea polymorpha, Microb Cell Fact., 2022, 13, 21, 162, doi: 10.1186/s12934-022-01889-z

128

Ruchala J, Andreieva YA, Tsyrulnyk AO, Sobchuk SM, Najdecka A, Wen L, Kang Y, Dmytruk OV, Dmytruk KV, Fedorovych DV, Sibirny AA. Cheese whey supports high riboflavin synthesis by the engineered strains of the flavinogenic yeast Candida famata, Microb Cell Fact., 2022, 13, 21, 161, doi: 10.1186/s12934-022-01888-0

129

Kit Y, Starykovych M, Manko N, Orfin A, Alexanyan T, Bozhko L, Turchyna T, Kit O, Krishnankutty R, Anand A, Sibirny A, Souchelnytskyi S, Stoika R. Elevation of truncated (48 kDa) form of unconventional myosin 1C in blood serum correlates with severe Covid-19, J Immunol Methods, 2023, 514,113437, doi: 10.1016/j.jim.2023.113437

130

Fedorovych DV, Tsyrulnyk AO, Ruchala J, Sobchuk SM, Dmytruk KV, Fayura LR, Sibirny AA. Construction of the advanced flavin mononucleotide producers in the flavinogenic yeast Candida famata,Yeast, 2023, 7, doi: 10.1002/yea.3843

131

Zazulya AZ, Semkiv MV, Stec M, Cyske Z, Gaffke L, Pierzynowska K, Węgrzyn G, Sibirny AA. The Komagatella phaffii ACG1 gene, encoding β-1,6-n-acetylglucosaminyltransferase, is involved in the autophagy of cytosolic and peroxisomal proteins, Yeast, 2023, 22, doi: 10.1002/yea.3846

132

Wang H, Narsing Rao MP, Cheng M, Xian M, Zhou Y, Zhou L, Cao H, Li WJ, Sibirny A, Wang , Liu Y, Li X, Kang YQ. Regulatory effect of moderate Jiang-flavour baijiu (Chinese liquor) dosage on organ function and gut microbiota in mice, J Biosci Bioeng, 2023, 11, S1389-1723(23)00001-4, doi: 10.1016/j.jbiosc.2023.01.001

1

Ruchala J, Kurylenko OO, Sibirny AA. Production of glutathione by yeasts and the role of this thiol in methylotrophic metabolism and xenobiotic detoxification, In: Sibirny A, Fedorovych D, Gonchar M, Grabek-Lejko D (eds), Living organisms and bioanalytical approaches for detoxification and monitoring of toxic compounds, University of Rzeszow in corporation with Institute of Cell Biology National Academy of Sciences of Ukraine, Rzeszow, 2015, 227-236, ISBN 978-83-7667-203-8

2

Kurylenko O, Ruchala J, Dmytruk K, Sibirny A. New targets for improvement of  xylose alcoholic fermentation in the methylotrophic yeast Hansenula polymorpha. In: Sibirny A, Fedorovych D, Gonchar M, Grabek-Lejko D (eds), Living organisms and bioanalytical approaches for detoxification and monitoring of toxic compounds. University of Rzeszow in corporation with Institute of Cell Biology National Academy of Sciences of Ukraine, Rzeszow. 2015, 247-257, ISBN 978-83-7667-203-8

3

Semkiv MV, Kurylenko OO, Ruchala J, Hryniv OB, Dmytruk KV, Sibirny AA. Yeast metabolic engineering for construction of the advanced bioethanol producers from renewable feedstocks In: Sibirny A, Fedorovych D, Gonchar M,  Grabek-Lejko D (eds), Living organisms and bioanalytical approaches for detoxification and  monitoring of toxic compounds, University of Rzeszow in corporation with Institute of Cell Biology National Academy of Sciences of Ukraine, Rzeszow, 2015, 333-341, ISBN 978-83-7667-203-8

4

Semkiv M, Kurylenko O, Ruchala J, Hryniv O, Kshanovska B, Dmytruk K, Sibirny A. Yeast alcoholic fermentation: achievements and challenges. In: Novikov V (eds), Modern directions in chemistry, biology, pharmacy and biotechnology, Lviv Politechnic National University, Lviv, Ukraine. 2015, 235-248, ISBN 978-617-607-824-1

5

Dmytruk K, Kurylenko O, Ruchala J, Ishchuk O, Sibirny A. Development of the thermotolerant methylotrophic yeast Hansenula polymorpha as efficient ethanol producer, In: Satyanarayana T, Kunze G (eds) Yeast Diversity in Human Welfare, Springer, Singapore, 2017, doi: 10.1007/978-981-10-2621-8_11

6

Dmytruk KV, Kurylenko OO, Ruchala J, Abbas CA, Sibirny AA. Genetic improvement of conventional and nonconventional yeasts for the production of  first- and second-generation ethanol, In: Sibirny A. (eds) Biotechnology of  Yeasts and Filamentous Fungi, Springer, Cham., 2017, doi: 10.1007/978-3-319-58829-2_1

Autorzy, tytuł wystąpienia, dane konferencji

Typ wykładu

1

International Congress on Yeasts, Madison, WI, USA, 2012

WZ

2

Molecular biology in infectious diseases and biotechnology, International conference, DiagMol, 2012, Warszawa, Polska

WZ

3

5 th R. Weigl Ukrainian-Polish conference, Chernivtsi, Ukraine, 38 th FEBS Congress, 2013, St. Petersburg, Rosja

WZ

4

30 th International Specialized Symposium on Yeasts, 2013, Stara Lesna, Słowacja

WZ

5

5 th FEMS Congress, 2013, Lepizig, Niemcy

WZ

6

26 th International Conference on Yeast Genetics and Molecular Biology, 2013, Frankfurt/Main, Niemcy

WZ

7

5 th Central European Congress on biotechnology, 2013, Kraków, Poland,

WZ

8

International workshop on High-temperature Fermentation Technology, 2014, Bangkok, Tajlandia

WZ

9

24 th International Conference on Arginine and Pyrimidines, 2014, Oxford, UK

WZ

10

7th International Weigl conference, 2015, Gdańsk, Polska

WZ

11

7th International Weigl conference, 2017, Lwów, Ukraina

WZ

12

33rd International Specilaized Symposium on Yeasts ISSY33, 2017, Cork, Irlandia

WZ

13

7th Congress of European microbiologists FEMS2017, 2017, Valencia, Hiszpania

WZ

14

“Non-conventional Yeasts: from Basic Research to Applications”, 2018, Rzeszow, Poland

WZ

15

“Advances in Microbiology and Biotechnology”, 2018, Lwów, Ukraina

WZ

16

4th Congress of Baltic Microbiologists, 2018, Gdańsk, Polska

WZ

17

8th International Weigl conference, 2019, Łódź, Polska

WZ

18

FEMS Conference, 2020, Belgrad, Serbia

WZ

19

The Congress of International Union of Microbiological Societies IUMS Seul, 2020, Płd. Korea

WZ

20

COST meeting on non-conventional yeasts (La Coruna, Spain, September 2021, Bari, Italy, 2022), ISSY36, 2022, Vancouver, Canada

WZ

21

Plenary Lecture at 32nd International Specialized Symposium on Yeasts, 2015, Perugia, Włochy

WP

22

Plenary Lecture at 14th International Congress on Yeasts, 2016, Awaji island, Japonia

WP

23

Biotechnology: Research and Insdustrial Applications, 2018, Wrocław, Polska

WP

24

35th International Specialized Symposium on Yeasts ISSY35, 2019, Antalya, Turcja

WP

25

27th International Conference on Yeast Genetics and Molecular Biology, 2015, Levico Terme, Włochy

Nazwa konferencji, miejsce, data

Funkcja

1

Non-conventional Yeasts in the Postgenomic Era, Lwów, Ukraina, 2011

Organizator, Przewodniczący komitetu organizacyjnego

2

27 th International Conference on Yeast Genetics and Molecular Biology, Levico, Trento, Włochy, 2015

Wice-przewodniczący komitetu organizacyjnego

3

6-ta Międzynarodowa Konferencja Młodych Naukowców „Human-Nutrition-Environment”, Rzeszów, Polska, 2016

Członek komitetu naukowego

4

International Conference Advances in Microbiology and Biotechnology, Lwów, Ukraina, 2018

Przewodniczący komitetu organizacyjnego

5

Non-conventional Yeasts: from Basic Research to Application, Rzeszów Polska, 2018

Przewodniczący komitetu organizacyjnego

6

1^st Polish Yeasts Conference, Rzeszów, Polska, 2022

Przewodniczący komitetu organizacyjnego, Przewodniczacy komitetu naukowego

7

R. Weigl’s International conferences, Lwów, Ukraina, 2004, Kijów, Ukraina, 2007,Yalta, Ukraina, 2012, Uzghorod, Ukraina, 2014, Odessa, Ukraina, 2016, Yaremche, Ukraina, 2019

Tytuł projektu, organizacja fundująca, okres trwania

Funkcja

1

Non-conventional yeasts for ethanol, chemical and food production: Next generation techniques for  manipulation and analysis, Visby, grant Instytutu Szwedzkiego (SI), 2012-2014

Kierownik

2

Metabolic engineering of the yeast Hansenula polymorpha for increase the level of glutathione, Ministerstwo Nauki i Szkolnictwa Wyższego, programu wymiany osobowej w ramach współpracy naukowej i naukowo-technicznej z Walonią, 2013-2014

Kierownik

3

Scientific integration of the Polish-Ukraine borderland area in the field of monitoring and detoxification of harmful substances in  environment, projekt współfinansowany przez Unię Europejską w ramach Programu Współpracy Transgranicznej Polska-Białoruś-Ukraina 2007-2013, 2013-2015

Kierownik

4

Zastosowanie inżynierii metabolicznej drożdży do  konstruowania ulepszonych producentów etanolu, NCN, 2013-2016

Kierownik

5

Klonowanie genów zaangażowanych w proces biosyntezy antybiotyku rozeoflawiny pochodzących od naturalnych producentów tego związku Streptomyces davawensis do komórek drożdży Candida famata i Pichia pastoris nadproducentów ryboflawiny, Ministerstwo Edukacji i Nauki, program wymiany osobowej w ramach współpracy naukowej i naukowo-technicznej z Austrią, 2016-2018

Kierownik

6

Rola czynników transkrypcyjnych w regulacji metabolizmu oraz fermentacji glukozy i ksylozy u niekonwencjonalnych drożdży Ogataea polymorpha, NCN, 2017-2022

Kierownik

7

Genetyczna kontrola metabolizmu oraz fermentacji alkoholowej pentoz (D-ksyloza, L-arabinoza) u termotolerancyjnych drożdży Ogataea polymorpha, NCN, 2019-2024

Kierownik

8

Mechanizmy regulatorowe zaangażowane w nadprodukcję ryboflawiny u flawinogennych drożdży Candida famata, NCN, 2019-2022

Kierownik

9

Konstruowanie oraz właściwości rekombinowanych drożdżowych szczepów produkujących bakteryjne antybiotyki rozeoflawinę oraz aminoryboflawinę, NCN, 2022-2025

Kierownik

Nr patentu

Autorzy, tytuł patentu, data przyznania

1

2

3

4

Imię i nazwisko

Tytuł rozprawy doktorskiej i jednostka

Rok

1

Mykola Maidan

Konstruowanie nowych bioelementów sensorów w celu oznaczania metanolu, etanolu oraz formaldehydu na bazie komórek drożdży metylotroficznych

1999

2

Andriy Voronovsky

Opracowanie systemu genetycznej transformacji dla flawinogennych drożdży Pichia guilliermondii i Candida famata

2001

3

Oleh Stasyk

Inżynieria genu GCR1 kontrolującego represję kataboliczną u metylotroficznych drożdży Hansenula polymorpha

2004

4

Taras Nazarko

Molekularny mechanizm degradacji peroksysomów u asymilujących alkany drożdży Yarrowia lipolytica

2005

5

Kostyantyn Dmytruk

Identyfikacja strukturalnych oraz regulatorowych genów biosyntezy ryboflawiny u flawinogennych drożdży Candida famata

2006

6

Olena Dmytruk

Inżynieria metaboliczna początkowych etapów katabolizmu ksylozy u drożdży Hansenula polymorpha w celu konstruowania ulepszonych producentów etanolu

2009

7

Dorota Grabek-Lejko

Opracowanie nowych metod analizy alkoholi i formaldehydu oraz selekcja mutantów drożdży ze zmienioną zdolnością do fermentacji alkoholowej

2010

8

Olena Ishchuk

Molekularne mechanizmy odporności do wysokiej temperatury oraz etanolu u drożdży niekonwencjonalnych

2010

9

Volodymyr Nazarko

Funkcjonalna rola białek zaangażowanych w peksofagię u drożdży

2011

10

Yurii Pynyaha

Opracowanie systemu genetycznej transformacji flawinogennych drożdży Pichia guilliermondii»

2011

11

Justyna Ruchała

Konstruowanie szczepów drożdży Hansenula polymorpha z ulepszonymi charakterystykami alkoholowej fermentacji ksylozy

2015

12

Maciej Kluz

Optymalizacja procesów produkcji ryboflawiny (witaminy B2) przez zrekombinowane szczepy drożdży Candida famata

2016

13

Olena Kurylenko

Metaboliczna inżynieria drożdży Ogataea polymorpha dla ulepszenia alkoholowej fermentacji ksylozy w podwyższonej temperaturze

2017

14

Marta Semkiv

Metaboliczna inżynieria drożdży Saccharomyces cerevisiae dla konstruowania efektywnych producentów etanolu paliwowego oraz glicerolu

2017

15

Iwona Kata

Konstruowanie szczepów drożdży Ogataea (Hansenula) polymorpha zdolnych do efektywnej konwersji glicerolu do etanolu

2018

16

Roxolana Vasylyshyn

Konstruowanie ulepszonych producentów etanolu z ksylozy u drożdży Hansenula polymorpha

17

Yana Petrovs’ka

Rola czynnika transkrypcyjnego Sef1p i kopijności strukturalnych genów w regulacji syntezy ryboflawiny u flawinogennych drożdży Candida famata

Imię i nazwisko

Tytuł rozprawy doktorskiej, postępowania o nadanie dr hab. lub tytułu profesora, jednostka

Rok

1

dr Znigniew Lazar

Doskonalenie metabolizmu cukrów w przemysłowych szczepach drożdży Yarrowia lipolytica, Uniwersytet Przyrodniczy we Wrocławiu

2017

2

dr Marcin Grąz

Rola kwasu szczawiowego w mechanizmie tolerancji metali przez Abortiporus biennis oraz wpływ na metabolizm grzyba, Uniwersytet Marii Curie-Skłodowskiej w Lublinie

2018

3

dr hab. Ewa Obłąk

Podstępowanie o nadanie tytułu profesora

2020

Członkostwo

Funkcja

1

Polskie Towarzystwo Mikrobiologów

Członek

2

COST (European Cooperation in Science and Technology), YEAST4BIO

Członek

2

Europejska Akademii Mikrobiologii

Członek

4

Federacja Europejskich Towarzystw Mikrobiologicznych

Dyrektor do spraw edukacji mikrobiologicznej

5

Narodowa Akademia Nauk Ukrainy

Rzeczywisty członek

6

Łotyska Akdemii Nauk

Zagraniczny członek